Products/Services Used | Details | Operation |
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Peptide Synthesis> | … My BioSource (San Diego, CA). Peptides. The following peptides were used: pepBBK07 (kindly provided by Dr. Utpal Pal, U. of Maryland) and PepVF (synthesized at GenScript, Piscataway, NJ). PepVF design: we modified a … | Get A Quote |
Currently, diagnostic testing for Lyme disease is done by determination of the serologic responses to Borrelia burgdorferi antigens, with the exception of the early localized phase of disease where diagnosis must be done clinically. Here, we describe the use of microfluidics technology to develop a multiplexed rapid lab-on-a-chip point of care (POC) assay for the serologic diagnosis of human Lyme disease. Following ELISA screening of 12 candidate antigens, we tested 8 on a microfluidic diagnostic system, called mChip-Ld, using a set of 60 serological samples. The mChip-Ld test, which can be performed in 15 minutes at the point of care, showed promising performance for detection of antibodies t... More